Dr. Salman Ahmed

Growth of brushite crystals on glass slide: a simple and inexpensive in vitro model to evaluate natural antiurolithiatic drugs

• Authors Details :  
• Salman Ahmed,  
• Muhammad Mohtasheemul Hasan

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The kidney contains mineral deposits in various phases of calcium salts, such as calcium oxalate and calcium phosphate. Calcium hydrogen phosphate dihydrate (CaHPO4.2H2O) / brushite is a stable form of calcium phosphate, which exists in the form of kidney and bladder stones. The study aimed to explore the possible morphological features of brushite crystals. The study was carried out on a glass slide under a microscope to observe the growth patterns of these crystals. For this purpose, a drop of gel medium (sodium meta silicate solution + 1M orthophosphoric acid solution) at pH 4.99-5.09 was placed in the middle of a glass slide and converted into a gel. A 1M calcium chloride drop was added to form the gel adequately. The glass slide was observed under a microscope till it was completely dried. Calcium hydrogen phosphate dihydrate was formed as elementary needles, needle clusters, an assemblage of needles with platy crystals, plates with spatial branches, radiating assemblage of platy crystals, star shape crystals and tetragonal bipyramidal. The Current study provides brushite crystallisation phases on glass slides for the first time. It may also be used as a model to evaluate prophylactic management against renal stones through in vitro assessment of crystal growth and aggregation inhibition and modulation of developing or developed crystals by using different natural products. However, it was a preliminary study with no quantitative or statistical analysis.

Article Subject Details

• Pharmaceutical sciences
• Pharmacognosy
• Pharmacology
• Pharmacology and toxicology
• Pharmacy

Article Keywords Details

• Brushite
• In vitro technique
• Kidney stone
• Medicinal plants for kidney stone
• Pharmacognosy
• Pharmacology
• Pharmacy

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