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The plant surface especially the leaf surface i.e., phyllosphere is exposed to dust and other particulates resulting in the establishment of a typical microflora. The surfaces of aerial plant parts provide habitat for epiphytic micro-organisms, many of which also influence the growth of pathogens. Microbial life in the phyllosphere is of great commercial importance to the agricultural industry for understanding the survival of plant disease-causing bacteria and fungi is vital for developing new ways to control their spread. In the present investigation, microflora isolated from the surface of a leaf of different medicinal, garden, terrestrial and aquatic plants. In this work fungus, bacteria and actinomycetes are isolated by using leaf washing methods on suitable nutritive media. Generally, the present work carried out by observation and identification of microflora under the microscope and biochemical test methods. The present work reveals that fifteen mycoflora (fungi), fifteen bacterial strains
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