Dr. Salman Ahmed
Near infra red spectroscopic analysis of macrotyloma uniflorum (lam.) verdc., phaseolus lunatus linn. and phaseolus vulgaris linn.
Journal title : Journal of Pharmaceutical & Scientific Innovation
Publisher : Annals of Medical and Health Science Research
Online ISSN : 2277-4572
Page Number : 23-26
Journal volume : 5
Journal issue : 1
62 Views Original Article
The present study is designed to determine the types of secondary metabolites in three selected seed powders using a fast, reliable, and non-destructive Near Infra Red spectroscopic analytical technique. NIRS analysis of Macrotyloma uniflorum (Lam.) Verdc., Phaseolus lunatus Linn., and Phaseolus vulgaris Linn., seed flours were used to qualitatively evaluate secondary metabolites. The fine powder of crude drug was directly applied to FOSS XDS near the infrared Rapid Content Analyzer. The adequately filled powdered sample in a disposable glass silicate vial closed with a plastic lid was centrally placed onto the Foss Rapid Content Analyzer sample presentation glass. The scanning of samples at ambient temperature gives NIR spectra within 30 seconds. The major proposed compounds in M. uniflorum were 3,4-dihydroxybenzoic acid, caffeic acid, chlorogenic acid, ferulic acid, gallic acid, kaempferol, linolenic acid, niacin, quercetin, sinapinic acid, syringic acid, and vanillic acid. Whereas coumaric acid, fructose, galactose, L-serine, raffinose, stachyose, sucrose, threonine, and tyrosine were detected in P. lunatus, and the NIR suggested the presence of caffeic acid, ferulic acid, sinapinic acid, phytic acid, leucine, and valine in P. vulgaris. NIRS is an advanced innovative technology extensively used in chemical, food, petrochemical, and pharmaceutical industries for rapid and reliable standardization. The present study revealed an excellent correlation to conclude that flour samples of all three legume seeds could be added to the food system to achieve nutritional and functional benefits.
Article DOI & Crossmark Data
DOI : https://doi.org/10.7897/2277-4572.0516
Article Subject Details
Article Keywords Details
- Macrotyloma
- Near infra red spectroscopy
- Pharmacognosy
- Pharmacy
- Phaseolus
- Phytochemical investigation
- Spectroscopy
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