Dr. Salman Ahmed
Inhibition of calcium oxalate crystals growth by macrotyloma uniflorum (lam.) verdc, phaseolus lunatus linn, and phaseolus vulgaris linn: an in vitro study
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Purpose: The study aims to explore all possible morphological features of calcium oxalate monohydrate and calcium oxalate dihydrate crystals and their habits in case of inhibition. Methods: The study was carried out on a glass slide to observe the growth and inhibition of calcium oxalate monohydrate (COM) crystals by using infusions (5-20%) of Macrotyloma uniflorum (Lam.) Verdc, Phaseolus lunatus Linn. and Phaseolus vulgaris Linn. The reagents of the double diffusion gel technique were used for this purpose. Results: Calcium oxalate crystals are divided into three types: calcium oxalate monohydrate, dihydrate, and trihydrate. These types are further divided into sub-types based on their morphology. In the case of calcium oxalate monohydrate (COM), these crystals are donuts, dumbbells, needles, platy, prismatic, rosette, round edges and X-shaped. Calcium oxalate dihydrate (COD) is the elongated large rods and tetragonal bipyramidal forms. In the present study, dendritic or arborescent (tree-like platy crystals) were observed for the first time as part of a COM growth. Long chain loose agglomerates and compact aggregated crystals are the typical pattern of calcium oxalate crystals. All tested infusions caused growth inhibition of calcium oxalate crystals. Smaller nucleation zones and defective shapes of the grown crystals are declared different growth inhibition patterns. Conclusion: This study gives extensive information about morphology, aggregation and growth inhibition of calcium oxalate crystals.
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