Dr. Salman Ahmed

Growth patterns of monosodium urate monohydrate (gouty and urinary) crystals in gel : an in vitro study

• Authors Details :  
• Salman Ahmed,  
• Muhammad Mohtasheemul Hasan ,  
• Zafar Alam Mahmood

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Monosodium urate monohydrate crystals are deposited in peripheral joints, causing gout and eliciting an intense localized inflammatory attack. In contrast, collecting ducts and medullary interstitium is a urinary calculus, which causes dysuria, nausea and hematuria. The purpose of the present study is to observe possible growth patterns of monosodium urate monohydrate crystals. The crystals were grown in test tubes using a single diffusion gel technique and were observed on the 7th,14th, 21st, 28th, and 40th days. Needle and spherulite-type crystals were observed on the 14th day, while arboresque, crystal sheaves, densely branched, dumbbell, mushroom-type spherulites, plumose and hexagonal prismatic crystals were observed for the first time. After the 40th day, complete spherulites were observed with their aggregates. The crystals were characterized by Scanning Electron Microscopy, Energy-Dispersive X-ray and Fourier Transform infrared spectroscopies. The pattern of MSUM will help determine the role of different natural products in the modulation, inhibition or promotion of these crystals by affecting the shape, size, transparency, approximate number and total mass of growing crystals. In case of inhibition or modulation, it will help evaluate prophylactic management. In contrast, the promotion of the crystal will give an idea about the risk factors of gout and kidney stones.

Article Subject Details

• Nephrology
• Pharmaceutical sciences
• Pharmacognosy
• Pharmacology
• Pharmacology and toxicology
• Pharmacy

Article Keywords Details

• Gout
• In vitro technique
• Kidney stone
• Medicinal plants for kidney stone
• Pharmacognosy
• Pharmacology
• Pharmacy

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